Below is a comparison of the protocols for recording electrocardiograms in awake mice. On the left is a partial description of the steps involved if using telemetric implants. On the right are the steps involved if using the ECGenie.
Species: Adult mic
|Step||Telemetric Implants 1,2||Step||ECGenie 1,3,4|
|1.||Remove fur from the surgical incision sites (Nair) and clean the exposed skin with Betadine followed by 70% alcohol.||1.||Gently place the mice on the ECGenie platform & allow them to acclimate for ~ 10 min.|
|1.||Anesthetize and place the mouse in the supine position, then make a ~ 1cm midscapular transverse incision on the back of the mouse.|
|2.||Using round tip scissors, undermine theskin on the left lower back to create a subcutaneous pocket large enough to implant the transmitter.|
|3.||Insert the transmitter device into the pocket with the side of the device containingthe predrilled suture holes facing up; fix it to the overlying skin with 5-0 nylon sutures.|
|4.||Close all incisions and openings with nylon sutures with the knots buried beneath the skin.|
|5.||Manage pain via a bolus injection of 0.1 mg/kg of buprenorphine subcutaneously.|
|6.||Return the animal to the cage and allow recovering or up to 3 weeks for it to return to its pre-surgical body weight.|
|7.||Power on the battery in the transmitter using a magnet. Verify the functioning of the transmitter via an AM radio placed close to the device and listen for a high-pitched tone that corresponds to the cardiac cycle.|
|8.||Place the signal reception plate under the animal cage and connect the cables to thedata acquisition system for recording, storage and analysis.|
|9.||Record ECG in Awake Mice||2.||Record ECG in Awake Mice|
- Ho D et al. Heart Rate and Electrocardiography Monitoring in Mice. Curr Protoc Mouse Biol. 2011 Mar 1;1:123-139.
- Georgetown University Animal Care and Use Committee Standard Procedure: Telemetry Unit Implantation in Mice and Rats
- Heier et al. Development of electrocardiogram intervals during growth of FVB/N neonate mice. BMC Physiol. 2010 Aug 24;10:16.
- Suidan et al. Lack of tryptophan hydroxylase-1 in mice results in gait abnormalities. PLoS One. 2013;8(3):e59032.